煙堿型乙酰膽堿受體(nAChRs)是一種配體門控離子通道受體，存在于中樞和周圍神經(jīng)系統(tǒng)的神經(jīng)元上以及神經(jīng)肌肉接頭內(nèi)的肌肉細(xì)胞上。nAChRs進(jìn)一步分為肌肉型（N1）和神經(jīng)元型（N2）兩種類型，其中肌肉煙堿受體功能是引發(fā)骨骼肌中的隨意肌肉運(yùn)動(dòng)。在與Ach結(jié)合后，煙堿受體特異性誘導(dǎo)效應(yīng)細(xì)胞的細(xì)胞膜上形成離子通道，帶正電的離子（陽(yáng)離子）可以通過(guò)該離子通道進(jìn)入細(xì)胞內(nèi)。離子的流入導(dǎo)致細(xì)胞膜去極化并產(chǎn)生動(dòng)作電位，例如觀察到的肌肉收縮。所以，肌肉型煙堿受體是眾所周知的肌肉松弛劑的靶點(diǎn)。

愛(ài)思益普電生理團(tuán)隊(duì)構(gòu)建了包括α1β1δε nAChR在內(nèi)的多種煙堿型乙酰膽堿離子通道細(xì)胞系以及不同通量的膜片鉗檢測(cè)方法，具有豐富的篩選經(jīng)驗(yàn)。自動(dòng)膜片鉗（APC）方便試驗(yàn)人員獨(dú)立操作，并且檢測(cè)通量高，我們使用SophionQPatch 48X可以自動(dòng)化滿足絕大多數(shù)離子通道的電生理檢測(cè)。

nAChRα1β1εδ數(shù)據(jù)展示-自動(dòng)膜片鉗

Figure 1. Acetylcholine is the endogenous agonist at muscarinic and nicotinic cholinergic receptors. (a) Dose-response curve ofacetylcholineon α1β1δε nAChR in Qpatch platform. (b)Representative nAChR current agonizedby multiple concentrations of acetylcholinein CHO cells expressing human α1β1δε.

Figure 2. Adiphenine is an acetylcholine receptor inhibitor used as an antispasmodic drug due to its strong smooth muscle relaxant action.(a) Dose-response curve ofadiphenineinhibition of 8 μM Ach induced α1β1δε nAChRcurrent in Qpatch platform.(b)Representative α1β1δε nAChR current traces induced by 8 μM AChbefore and after application with multiple concentrations ofadiphenine.